rabbit anti-mouse yap1 d8h1x  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: Yap1-Driven Intestinal Repair Is Controlled by Group 3 Innate Lymphoid Cells.

doi: 10.1016/j.celrep.2019.11.115

Figure Lengend Snippet: Figure 2. YAP1 Activation Drives Acute Small Intestinal Regeneration (A) Fragments per kilobase of exon per million fragments mapped (FPKM) values of Yap1 target genes in LGR5-GFPhi crypt cells analyzed by RNA sequencing at steady state (SS), one and four days after MTX.

Article Snippet: Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X, Cell signaling).

Techniques: Activation Assay, RNA Sequencing

Journal: Cell reports

Article Title: Yap1-Driven Intestinal Repair Is Controlled by Group 3 Innate Lymphoid Cells.

doi: 10.1016/j.celrep.2019.11.115

Figure Lengend Snippet: Figure 3. YAP1 Activation Is Blunted in the Absence of ILC3s (A) Representative YAP1 immunostaining of duodenal crypts from RORgt/ mice and littermate controls one day after MTX. Scale bar: 50 mm. (B) Percentage of duodenal crypts containing at least 1 cell with nuclear translocation of YAP1 in RORgt/ mice and littermate controls one day after MTX. (C–G) Log2 fold change values determined by RNA sequencing of Lgr5-GFPhi crypt cells comparing MTX day 1 versus SS for control mice (black bars), RORgt/ mice (red bars) and MTX day 4 versus SS for control mice (gray bars), or RORgt/ mice (blue bars) showing (C) YAP1 target genes, (D) Wnt-related genes, (E) Notch-related genes, (F) intestinal stem cell genes, and (G) secretory cell genes. (H) Percentages of EECs determined by flow cytometry in duodenal crypts at the indicated time points from RORgt/ mice (red bars). Control mice (as in Figure 2G) are shown for comparison (black bars). EECs were gated as Live(+)CD45()Ter119()CD31()EpCAM1(+)Lgr5-GFP()CD24hiSSClo cells. Log2 fold change (DESeq2 analysis of count data) with **adjusted p < 0.01, *adjusted p < 0.05, or statistically not significant (not indicated) (C–F). FPKM values are plotted for transcripts that have statistically significant log2 fold change (DESeq2 analysis of count data) with **adjusted p < 0.01, *adjusted p < 0.05, or statistically not significant (not indicated). Unpaired Mann-Whitney test, *p < 0.01; statistically not significant (not indicated) (B and H). Error bars: SEM. n = 4–8 mice per group (A, B, and H), n = 3 mice per group (C–G). See also Figure S3.

Article Snippet: Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X, Cell signaling).

Techniques: Activation Assay, Immunostaining, Translocation Assay, RNA Sequencing, Control, Cytometry, Comparison, MANN-WHITNEY

Journal: Cell reports

Article Title: Yap1-Driven Intestinal Repair Is Controlled by Group 3 Innate Lymphoid Cells.

doi: 10.1016/j.celrep.2019.11.115

Figure Lengend Snippet: Figure 4. YAP1 Activation Involves gp130- SFK Signaling (A) FPKM values of gp130 and gp130 co-receptors transcribed by Lgr5-GFPhi crypt cells at steady state and one day after MTX. (B) FPKM values and relative expression by qPCR of gp130 ligands transcribed by intestinal CCR6+NKp46 ILC3s at steady state. (C) Representative YAP1 immunostaining and percentage of duodenal crypts containing at least 1 cell with nuclear translocation of YAP1 one day after MTX treatment in the presence of LMT-28 or vehicle control. (D) Representative flow cytometry plots and fre- quency of Lgr5-GFP+ cells in duodenal crypts four days after MTX from Lgr5-GFP+/ mice treated with STATTIC or DMSO vehicle control. Numbers indi- cate the percentage of Lgr5-GFP+ cells within Live(+)CD45()Ter119()CD31()EpCAM1(+)cells. (E) Representative Ki67 immunostaining and number of Ki67+ cells in duodenal crypts four days after MTX in Lgr5-GFP+/ mice treated with STATTIC or DMSO vehicle control. (F) Representative YAP1 immunostaining in duodenal crypts from mice treated with SFK in- hibitor PP2 or DMSO control one day after MTX. (G) Representative H&E and Ki67 staining of duodenal sections four days after MTX treatment in the presence of PP2 or DMSO control. (H) Crypt pathology score of small intestinal sec- tions four days after MTX in mice treated with PP2 or DMSO control. FPKM values are plotted for transcripts that have statistically significant log2 fold change (DESeq2 analysis of count data). Unpaired Mann-Whitney test, *p < 0.01 (B); unpaired t test, *p < 0.05(C and D). Errorbars:SEM.Scale bars:100mm (DandF),50mm (B); n = 3 mice per group (A, C, and D), n = 4–6 mice per group (B and E–G). See also Figure S4.

Article Snippet: Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X, Cell signaling).

Techniques: Activation Assay, Expressing, Immunostaining, Translocation Assay, Control, Cytometry, Staining, MANN-WHITNEY

Journal: Translational Lung Cancer Research

Article Title: SRC and PIM1 as potential co-targets to overcome resistance in MET deregulated non-small cell lung cancer

doi: 10.21037/tlcr-20-681

Figure Lengend Snippet: Primary and secondary antibodies used in the study

Article Snippet: Primary antibodies and secondary antibodies used are in . table ft1 table-wrap mode="anchored" t5 caption a7 Western blotting primary antibody Dilution Company and catalog number Mouse anti-beta-actin 1:5,000 Sigma Aldrich (#A5441) Rabbit anti-AKT 1:1,000 Cell signaling (#9272) Rabbit anti-Phospho-AKT (S473) 1:1,000 Cell signaling (#9271) Rabbit anti-AXL 1:1,000 Cell signaling (#8661) Rabbit anti-CDCP1 1:1,000 Cell signaling (#4115) Rabbit anti-Phospho-CDCP1 (Y707) 1:1,000 Cell signaling (#13111) Rabbit anti-EGFR 1:1,000 Cell signaling (#4267) Rabbit anti-Phospho-EGFR (Y1068) 1:1,000 Cell signaling (#3777) Rabbit anti-Phospho-EGFR (Y845) 1:1,000 Cell signaling (#6963) Rabbit anti-ERK1/2 1:1,000 Cell signaling (#9102) Rabbit anti-PhosphoERK1/2 (T202/Y204) 1:1,000 Cell signaling (#9101) Rabbit anti-cMET 1:1,000 Cell signaling (#8198) Rabbit anti-Phospho-cMET (Y1234-1235) 1:1,000 Cell signaling (#3077) Rabbit anti-Src 1:1,000 Cell signaling (#2109) Rabbit anti-Phospho-Src Family (Y416) 1:1,000 Cell signaling (#6943) Mouse anti-STAT3 1:1,000 Cell signaling (#9139) Rabbit anti-Phospho STAT3 (Y705) 1:1,000 Cell signaling (#9145) Mouse anti-YAP1 1:1,000 Cell signaling (#12395) Rabbit anti-Phospho YAP1 (Y357) 1:1,000 Abcam (#ab62751) Rabbit anti-AXL 1:1,000 Cell signaling (#8661) Donkey anti-Rabbit IgG HRP-linked 1:5,000 GE Healthcare (#NA934) Sheep anti-Mouse IgG HRP-linked 1:5,000 GE Healthcare (#NXA931) Open in a separate window caption a8 Primary and secondary antibodies used in the study Cell viability assay The EBC-1 human squamous lung cancer cell line with MET amplification, the Hs746T human gastric cancer cell line with MET exon 14 skipping mutation, and the H1993 human lung adenocarcinoma cell line harboring MET amplification were purchased from American Type Culture Collection (ATCC).

Techniques: